九九精品在线,可以在线观看的欧美黄片,美女张开腿让男生捅日本,日本人妻中文字幕高清不卡

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>HTB-148H4細胞,人腦神經(jīng)膠質瘤細胞

H4細胞,人腦神經(jīng)膠質瘤細胞

簡要描述:H4細胞,人腦神經(jīng)膠質瘤細胞
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:HTB-148
  • 廠商性質:生產(chǎn)廠家
  • 更新時間:2025-06-28
  • 訪  問  量:1877

產(chǎn)品分類

Product Category

詳細介紹

H4細胞,人腦神經(jīng)膠質瘤細胞

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4Depositors: J RiggsBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brain Disease: neurogliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. H4細胞,人腦神經(jīng)膠質瘤細胞Applications:transfection host (Roche FuGENE® Transfection Reagents)Tumorigenic:NoDNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,9 TPOX: 8,11 vWA: 14,18Cytogenetic Analysis:modal number = 73; range = 63 to 78.This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 1Age:37 yearsGender:maleEthnicity:CaucasianPropagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:             Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:454402622907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
黄色暴力视频免费一区二区三区| 国产V一区二区| 亚洲久天堂| 亚洲AV无码成人精品区亚非| 国产精品人妻无码18| 日本电影人人妻| 天天射天天干iⅤ| 久久久中文字幕日本无吗| 尤物后插视频| 欧美国内精品推荐综合| 欧美大香蕉成年女人看片新版| 成,亚洲视频| 美女av搞黄| 一区二区三区在线不卡av影院| 欧美日韩高新一区二区| 中文字幕精品视频一区二区三区| 国精一区二区三区千禧| 亚洲天堂无码狠狠久久| 人妻酷刑三区| 意大利法国精品一区二区| 欧美在线一区二区三区免费| 台湾姥中文娱乐网偷拍| 国产精品一区二区午夜视频| 日韩又大又粗又爽视频| a级精品久久| 欧美国产日韩小说一区| 日韩91一区不卡| 69精品在线观看| 大几把艹骚逼视频| 久久在线这里都是精品店| 色黄麻豆91AV视频| 日本韩国欧美不卡| 成人电影区| 天天干天天射天天操。| 国产伦一区二区三区免费Ai| av卡中文字幕| 日韩色aⅤ| 经典色呦呦| 亚洲无码SWAG在线| 色狠狠色噜噜aⅴ天堂一区| 三级在线www|