九九精品在线,可以在线观看的欧美黄片,美女张开腿让男生捅日本,日本人妻中文字幕高清不卡

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CCL-138Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

簡要描述:Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CCL-138
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-07-08
  • 訪  問  量:1834

產(chǎn)品分類

Product Category

詳細(xì)介紹

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

OrganismHomo sapiens, human
Tissuepharynx: derived from metastatic site: pleural effusion
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseasepharyngeal carcinoma
Age*****
Genderfemale
EthnicityCaucasian
Storage Conditionsliquid nitrogen vapor phase

 Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Caucasian

female

 Genes Expressed  

keratin

 Virus Susceptibility   Human poliovirus 1
Vesicular stomatitis virus              
 Comments  

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing





Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C              CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
AV高潮亚洲| 亚洲AV成人不卡电影在线| 香蕉伊热19| 在线观看五码视频| 风流老熟女一区二区三区aⅴ| 欧美日韩午夜视频免费观看| 青青草原国产在线视频,| 国产精品第75页| 76av导航| 亚久久久久| 日韩国产第一页第二页第三页| 中文字幕在线无码观看视频| 日韩人妻制服丝袜资源| 日本性感中文字幕| 熟妇 五十路一区| 百无码一区二区| 国产一区二区三区在线观看日本| 直接看欧美黄色片| 91小视频尤物| 黄频在线免费在线观看| 91豆花麻豆| 啪啪后入式动态一区二区| 男人的天堂久久久| 中文字幕久久精品北条麻妃96| 亚洲黄毛片| 黄片一级一区二区懂| 欧美一区二区图片小说| 亚洲色欲激情| AAA久久久利人佳欧美| 成人中文字幕二区| 国产精品久久精品毛片| 78色国产精品| 日本一区二区三区视频免费大全| 91免费在线美女| 人久久久久久久久国产电影| 亚洲图片噜在线看| 五月天 黄片| 中文字幕久久。| 日本高潮色哟哟| 日本午夜精品理论| 人妻内射中出电影|